Generating kidney organoids from human pluripotent stem cells using defined conditions

SE Howden; MH Little

Book Chapter

Generating kidney organoids from human pluripotent stem cells using defined conditions

SE Howden, MH Little

Methods in Molecular Biology | Methods in Molecular Biology | HUMANA PRESS INC | Published : 2020

DOI: 10.1007/978-1-0716-0655-1_15

Abstract

The ultimate goal of regenerative medicine is to have access to an unlimited supply of specific cell types on demand, which can be used as effective therapies for a wide range of intractable disorders. With the availability of human pluripotent stem cells (hPSCs) and greatly improved protocols for their directed differentiation into specific cell types, including kidney, this prospect could soon become a reality. We have previously described the generation of kidney organoids from hPSCs. This chapter describes our latest differentiation protocol for generating kidney tissue, which uses a cost-effective and completely defined, xeno-free medium. As with our previous protocol, these complex, mu..

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University of Melbourne Researchers

Little, MH Author

Related Projects (1)

Studying early human kidney development using stem cells

This project aims to improve our understanding of cell types, lineage relationships, cell-cell interactions and morphogenetic processes in h..

Grants

Awarded by National Institutes of Health

Funding Acknowledgements

This work was supported by the National Institutes of Health (DK107344), the National Health and Medical Research Council, Australia (GNT1100970) and the Australian Research Council (DP190101705). M.H.L is a Senior Principal Research Fellow of the NHMRC (GNT1136085). The Murdoch Children's Research Institute is supported by the Victorian Government's Operational Infrastructure Support Program.